UsetheCFX96DeepWellReal-TimePCRDetectionSystemforprecise,reproducIBLereal-timePCR.ThesystemiscomposedoftheCFX96DeepWellOpticalReactionModuleandtheC1000TouchThermalCyclerChassis.ThemoduleisamemberofthefamilyofinterchangeablereactionmodulesthatcanbeusedwiththeC1000TouchThermalCyclerChassisforPCRandreal-timePCR.Allmodulescanbequicklyinsertedandremovedfromthecyclerchassiswithouttools.
TheCFX96DeepWellReal-TimePCRDetectionSystemhassixindependentlycontrolledthermalelectricunitsprovidingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).
Theopticalsystem,withsixfilteredLEDsandsixfilteredphotodiodes,collectsdatafromallwells,detectinguptofivetargetsperwell.Forsingle-colorFAMandSYBR®GreenI,thefastscanoptionreadssingle-channelfluorescenceinall96wellsinjust3seconds.AchannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
Thelargetouch-screeninterfacehasintuitivegraphicalprogramminganddisplaysdatatracesinrealtime.Theprogrammablethermalgradientfeaturecantesteighttemperaturesatoncetoquicklydeterminetheoptimalannealingtemperature,andtheprotocolautowriterquicklyoptimizesreactions,generatinganoptimalprotocolbasedonyourpolymerase,primers,andproductlength.
ThefollowingmodulescanbeusedwiththeC1000TouchThermalCyclerChassis:
ReactionModules | CatalogNumber | |
Module | ModulewithStarterPackage* | |
ForPCR | ||
Dual48/48FastReactionModule | 1840148 | — |
96-WellFastReactonModule | 1840196 | — |
96–DeepWellReactionModule | 1840197 | — |
384-WellReactionModule | 1840138 | — |
ForReal-TimePCR | ||
CFX96OpticalReactionModule | 1845097 | 1845096 |
CFX96DeepWellOpticalReactionModule | 1844095 | 1844096 |
CFX384™OpticalReactionModule | 1845385 | 1845384 |
*StarterpackagecontainsSsoAdvanced™UniversalSYBR®GreenSupermix(1725270),Microseal®"B"AdhesiveSeals(MSB1001),andHard-Shell®Low-ProfileThin-Wall96-WellSkirtedPCRPlates(HSP9601)orHard-ShellThin-Wall384-WellSkirtedPCRPlates(HSP3805).
TheCFX96DeepWellReal-TimePCRDetectionSystemcanbeconfiguredwithotherPCRinstruments,allowingstand-aloneorsoftwarecontrol.InstrumentpermutationsallowuptofourdifferentCFXConnect™,CFX96,orCFX384SystemstobecontrolledbyonePC.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
ThermalCycler | |
Chassis | C1000Touch™ |
Maximumramprate,°C/sec | 2.5 |
Averageramprate,°C/sec | 2 |
Heatingandcoolingmethod | Peltier |
Lid,°C | Heatsupto105 |
Temperature | |
Range,°C | 0–100 |
Accuracy,°C | ±0.2ofprogrammedtargetat90°C |
Uniformity,°C | ±0.4well-to-wellwithin10secofarrivalat90°C |
Gradient | |
Operationalrange,°C | 30–100 |
Programmablespan,°C | 1–24 |
OpticalDetection | |
Excitation | 6filteredLEDs |
Detection | 6filteredphotodiodes |
Rangeofexcitation/emissionwavelengths,nm | 450–730 |
Sensitivity | Detects1copyoftargetsequenceinhumangenomicDNA |
Dynamicrange | 10ordersofmagnitude |
ScanTime | |
Allchannels,sec | 12 |
FAM/SYBR®Greenonly,sec | 3 |
Software | |
Operatingsystems | WindowsXP,Windows7 |
Multiplexanalysis | Upto5targetsperwell |
System | |
Licensedforreal-timePCR | Yes |
Samplecapacity,wells | 96 |
Samplesize,µl | 10–125 |
Communicationinterface | USB2.0 |
Electricalapprovals | IEC,CE |
Dimensions(WxDxH),cm/in | 33x46x36/13x18x14 |
Weight,kg/lb | 21/47 |
成立日期:1952年Bio Rad伯乐
首席执行官:Norman Schwartz
总公司地址:Hercules, California
经营范围:生命科学研究和临床诊断
联合销售额:21亿美元 (2014年)
全球员工人数:7600名
Bio Rad公司成立于1952,公司服务于生命科学研究和临床诊断的客户通过其全球网络,业务遍及全球。在它的存在,Bio-Rad公司已建立的客户关系,提高公司的研发力量长期支持创新的产品和解决方案,加快发现过程的介绍和医疗水平的提高。
生命科学
生命科学集团开发、生产,并提供广泛的实验室仪器、设备、耗材和试剂,在功能基因组学研究领域,越来越多的应用蛋白质组学、药物研发和制造、细胞生物学、食品安全与科学教育。该组排名前五的生命科学公司之一,是著名的质量,创新,并成功的为客户长期以来的关注。这些产品都是基于用于分离、纯化、鉴定、分析技术、生物材料和放大,如蛋白质、核酸、细胞和细菌。技术和应用包括电泳、成像、多重免疫分析、色谱、微生物学、生物信息学、蛋白质功能分析,转染,流式细胞仪、放大和实时数字PCR。Bio-Rad公司的产品支持人员在全世界的实验室。
临床诊断
临床诊断集团开发,制造,销售,并支持大量的医学筛查和诊断产品组合。公司是一家领先的专业诊断公司和它的产品是公认的糖尿病监测和质量控制系统的金标准。本集团亦以其血液病毒检测、血型、自身免疫性疾病和遗传性疾病的检测,和基于互联网的软件产品。Bio-Rad的临床诊断产品将广泛用于检测,识别和量化技术,在体液和组织中的物质。结果是用来帮助支持医疗诊断、检测、评价和监测和治疗疾病和其他医疗条件。